Composite

Part:BBa_K1321324:Design

Designed by: Michael Florea   Group: iGEM14_Imperial   (2014-10-08)

pLux01


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 4785
    Illegal SpeI site found at 1046
    Illegal PstI site found at 1929
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4785
    Illegal SpeI site found at 1046
    Illegal PstI site found at 1929
    Illegal NotI site found at 1922
    Illegal NotI site found at 4791
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4785
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 4785
    Illegal SpeI site found at 1046
    Illegal PstI site found at 1929
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 4785
    Illegal XbaI site found at 4800
    Illegal SpeI site found at 1046
    Illegal PstI site found at 1929
    Illegal NgoMIV site found at 3104
    Illegal AgeI site found at 1625
    Illegal AgeI site found at 1737
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 987


Design Notes

Source

References